Southern Blot Analysis

£115.00

695

This experiment introduces your students to Southern blotting as a tool for "DNA Fingerprinting" in a hypothetical paternity determination. DNA fragments are first separated by agarose gel electrophoresis, then transferred to a nylon membrane and finally visualized by staining.

• Explore genetics and Southern blotting while solving a paternity case
• Run electrophoresis on four DNA samples pre-digested with a restriction enzyme
• Visualize the results using a modified Southern blot
• Analyze DNA RFLP patterns from mother, child, and potential fathers to resolve the case
• Our modified southern blot procedure is fast, safe, and designed for the classroom

Kit Includes:

Instructions, DNA samples for electrophoresis, practice gel loading solution, UltraSpec-Agarose™, electrophoresis buffer, pipets, 5 pre-cut nylon membranes, 5 pre-cut blotting filter papers, Blue-Blot DNA Stain™.

All You Need:

Electrophoresis apparatus, power supply, 65º C Water bath, DNA visualization system, staining net & tray, automatic micropipettes, lab glassware, microwave or hot plate, distilled water, NaCl, NaOH, concentrated HCl, plastic wrap, forceps

*PLEASE NOTE WE ONLY SUPPLY CHEMICALS TO EDUCATIONAL ESTABLISHMENTS *

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Southern Blot Analysis

£115.00

£115.00

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